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| Bleaching of mutated cannabinoid receptor 1-GFP, freely diffusing in the cytoplasm of human astrocytes (U138MG). Pulse duration 50 ms with laser line 488, ROI diameter 5 μm, Plan-Apochromat 63x/1.40 oil. Birgit Kraus, Institute for Pharmaceutical Biology, Regensburg, Germany |
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| DirectFRAP in combination with Cell Observer® SD (spinning disc). Bleaching of flottilin-2 EGFP in HeLa cells. Pulse duration 200 ms with laserline 488, ROI diameter 7 μm, Plan-Apochromat 63x/1.40 oil. E. May, Bioimaging Center, University of Constance, Germany |
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| DirectFRAP in combination with Cell Observer® SD (Spinning Disc). Bleaching of EB3-Emerald (green) in LLC-PK1 cells, second staining: H2B-Cherry (red). Pulse duration 300 ms with laser line 488, ROI diameter 9,6 μm, C-Apochroma t 63x/1.20 W Korr UV-VIS-IR. Scott Olenych, DHMRI, Kannapolis, USA. |
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| Conversion of cytoplasmic tdEos in HeLa-Cells. The converted state is shown. Laserpulses with laser line 405, pulse duration 400 ms. Roberto Villasenor, Max-Planck-Institut für Molecular Cellbiology and Genetics, Germany. |
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