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| New Applications for Cell Research |
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Typical questions you clarify with DirectFRAP:
- Are there velocity limiting factors in diffusion? Are there interactions of the target protein with macromolecules?
- Are there local and temporal variations in the architecture/composition of the cell compartment under consideration (spatial/temporal mapping)?
- What happens subsequent to photo manipulation of small cell structures? Does recovery occur at all; is there a directional dependence?
- From which compartment do the fusion proteins which are responsible for recovery come from?
- What are the diffusion coefficients and what is the size of protein complexes? What is the viscosity?
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 DirectFRAP in combination with Cell Observer® SD.
Bleaching of flottilin-2 E GFP (membrane-bound) in HeLa cells.
Impulse duration 200 ms with Laserline 488, ROI diameter 7 µm,
Plan-Apochromat 63x/1.40 Oil.
E. May, Bioimaging Center, University of Constance, Germany |
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DirectFRAP in combination with Cell Observer® SD.
Bleaching of flottilin-2 E GFP (membrane-bound) in HeLa cells.
Impulse duration 200 ms with Laserline 488, ROI diameter 7 µm,
Plan-Apochromat 63x/1.40 Oil.
E. May, Bioimaging Center, University of Constance, Germany |
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