Lightsheet workshop

Microscopy Course

Fluorescence Correlation Spectroscopy
in Biomedical Research

Fluorescence Correlation Spectroscopy

in Biomedical Research

Duration: 2 days, 9 am – 5:30 pm
Participants: min. 4, max. 6
Course Fee: 1020.00 EUR, for a three day course excl. tax
Course material and lunch are included
Prerequisites: Basic knowledge in light microscopy and fluorescence microscopy. Basic knowledge in Laser Scanning Microscopy is recommended.
General: Fluorescence Correlation Spectroscopy (FCS) is a highly sensitive method that detects movement and binding of fluorescence-labelled particles on the single molecule level.

Furthermore concentrations of movable fluorescent particles can be esti-mated. Thus biochemical reactions can be monitored in real time solutions as well as within living cells.

This course will familiarize you with the theoretical background of FCS and train you how to perform and evaluate measurements in solutions and living cells.
Topics: - Theory of FCS
- The correlation function
- Model functions for diffusion processes
- Data acquisition for FCS
- Data analysis for FCS
- APD imaging
- Objective lenses for FCS and FCCS

All hands on will be exercised on LSM 7-generation systems (LSM 710 with ConfoCor 3, LSM 780, LSM BiG) using the ZEISS ZEN software.

If you have further questions, please contact:

Dr. Christian Hellriegel
E-mail: christian .hellriegel @zeiss .com
Phone: +49 3641 64 2677
Fax:     +49 3641 64 3144

Lydia Engel
E-mail:  Lydia .Engel @zeiss .com
Phone: +49 3641 64 3653
Fax:     +49 3641 2847031

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