Lightsheet workshop

Microscopy Workshop

Laser Scanning Microscopy Introduction to Biomedical Applications

LSM 700

Biomedical Applications

Duration: 2 days, 9 am – 5:30 pm
Participants: min. 3, max. 4
Course Fee: 1020.00 EUR, for a two day course excl. tax
Course material and lunch are included
Prerequisites: Basic knowledge in light microscopy
General: Laser Scanning Microscopy is a standard method in biomedical research. Excellent knowledge of its theory and application is a prerequisite for experimental success.
This course provides detailed knowledge about point scanning confocal mi-croscopes in respect to its assembly, features, and application in biomedical sciences.
Topics:

- Principles of point scanning LSM

  • Beam paths and configurations of the LSM 700
  • Variable Secondary Dichroic Beamsplitter (VSD)

- Emission filters and avoiding Crosstalk
- Image acquisition and optimization
- Multi fluorescent imaging and channel linear unmixing
- Acquisition of Lambda stacks and Linear unmixing
- Basic image analysis and Colocalization
- 3D image acquisition and reconstruction
- System maintenance and care (SMT, ConfigTool, MTB)
- Objective lenses selection for Laser Scanning Microscopy

All hands on will be exercised on LSM 7-generation systems (LSM 700) using the ZEISS ZEN software.

If you have further questions, please contact:
E-mail:
  courses .microscopy .de @zeiss .com
Phone: +49 3641 64 3653
Fax:      +49 3641 2847031